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Catalog Number | orb1252496 |
---|---|
Category | Antibodies |
Description | KRT18 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | C-04 or Ks18.04 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Bovine, Canine, Equine, Feline, Ferret, Goat, Hamster, Human, Marmoset, Mouse, Porcine, Rat, Sheep |
Isotype | IgG1, kappa |
Immunogen | A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Keratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | KRT18 |
UniProt ID | P05783 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Keratin, type I cytoskeletal 18, Cell proliferatio Read more... |
Note | For research use only |
Application notes | Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Keratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Keratin 18 antibody (C-04).
Western blot testing of A431 cell lysate with Keratin 18 antibody (clone C-04).
SDS-PAGE Analysis of Purified, BSA-Free Keratin 18 Antibody (clone C-04 or Ks18.04). Confirmation of Integrity and Purity of the Antibody.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Canine, Hamster, Human, Rat, Sheep | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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