KRT10 Antibody / Cytokeratin 10

• Catalog Number: orb639773
• Category: Antibodies
• Description: This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AE20
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: Human Cytokeratin 10 was used as the immunogen for this Cytokeratin 10 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 10 antibody is available for research use only.
• Storage: Store the Cytokeratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skin with Cytokeratin 10 antibody (clone AE20). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 10 antibody (clone AE10) as confirmation of integrity and purity.