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    KPNA4 antibody

    Catalog Number: orb340814

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb340814
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to KPNA4
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human Karyopherin alpha-4
    Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetKPNA4
    Entrez16649, 3840
    UniProt IDO00629, O35343
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesQIP1; Importin subunit alpha-3; Importin alpha Q1;
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    KPNA4 antibody

    Western blot analysis of Karyopherin alpha-4 expression in A549 (A), SKOV3 (B) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 58 kD)

    KPNA4 antibody

    Immunohistochemical analysis of Karyopherin alpha-4 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    KPNA4 antibody

    Immunofluorescent analysis of Karyopherin alpha-4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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      ICC,  IF,  IHC,  WB

      Human, Mouse, Rat

      Polyclonal

      Unconjugated

      100 μl, 200 μl, 50 μl
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      ICC,  IHC-P,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
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      ELISA,  IF,  IHC,  WB

      Human, Mouse

      Rabbit

      Polyclonal

      Unconjugated

      50 μg, 100 μg
    • KPNA4 antibody [orb18608]

      ELISA,  WB

      Bovine, Canine, Human, Mouse, Rat

      Goat

      Polyclonal

      Unconjugated

      100 μg
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      ELISA,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
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