KNTC1 Rabbit Polyclonal Antibody

• Catalog Number: orb101601
• Category: Antibodies
• Description: KNTC1 Rabbit Polyclonal Antibody
• Target: KNTC1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human KNTC1/Kinetochore (1881-1980/2209aa)
• UniProt ID: P50748
• MW: 251 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HsROD; Kinetochore associated 1; KNTC1; ROD; Rough
• Note: For research use only

References

Huang, Houfeng et al. Knockdown of KNTC1 Inhibits the Proliferation, Migration and Tumorigenesis of Human Bladder Cancer Cells and Induces Apoptosis Crit Rev Eukaryot Gene Expr, 31, 49-60 (2021)

PubMed: 33639055

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KNTC1) Polyclonal Antibody, Unconjugated (orb101601) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.