KMT2D/ALL1 Rabbit Polyclonal Antibody

SKU: orb184541

Description

KMT2D/ALL1 Rabbit Polyclonal Antibody

Research Area

Cell Biology, DNA and RNA, Epigenetics and Nuclear Signaling, Trithorax-group proteins (TrxG)

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KMT2D/ALL1 (1301-1400/5537aa)
• Target: KMT2D
• Molecular Weight: 593 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AAD10; ALL1 related gene; ALL1-related protein; ALR; CAGL114; Histone-lysine N-methyltransferase MLL2; KABUK1; Kabuki make up syndrome; Kabuki mental retardation syndrome; KMS; KMT2D; Lysine N methyltransferase 2D; MLL2; KMT2D_HUMAN; MLL4; Myeloid/lymphoid or mixed lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; TNRC21; Trinucleotide repeat containing 21.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KMT2D/ALL1) Polyclonal Antibody, Unconjugated (orb184541) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

KMT2D

UniProt

O14686

References

Marco Seehawer 1 2 3, Zheqi Li 1 2 3, Jun Nishida 1 2 3, Pierre Foidart 1 2 3, Andrew H Reiter 4, Ernesto Rojas-Jimenez 1 2 3, Marie-Anne Goyette 1 2 3, Pengze Yan 1 2 3, Shaunak Raval 4, Miguel Munoz Gomez 5, Paloma Cejas 5, Henry W Long 5, Malvina Papanastasiou 4, Kornelia Polyak Loss of Kmt2c or Kmt2d drives brain metastasis via KDM6A-dependent upregulation of MMP3 Nat Cell Biol, (2024)

PubMed: 38926506

Applications

WB

Reactivity

Mouse

Cuiyuan Huang et al. Down-regulation of KMT2D Mitigates Neointimal Hyperplasia Following Carotid Artery Injury in Diabetic Rats Biochemical and Biophysical Research Communications, (2025)

PubMed: