KMT2D/ALL1 Rabbit Polyclonal Antibody

• Catalog Number: orb184541
• Category: Antibodies
• Description: KMT2D/ALL1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rat, Sheep
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KMT2D/ALL1 (1301-1400/5537aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 593 kDa
• Target: KMT2D
• UniProt ID: O14686
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AAD10; ALL1 related gene; ALL1-related protein; AL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Marco Seehawer 1 2 3, Zheqi Li 1 2 3, Jun Nishida 1 2 3, Pierre Foidart 1 2 3, Andrew H Reiter 4, Ernesto Rojas-Jimenez 1 2 3, Marie-Anne Goyette 1 2 3, Pengze Yan 1 2 3, Shaunak Raval 4, Miguel Munoz Gomez 5, Paloma Cejas 5, Henry W Long 5, Malvina Papanastasiou 4, Kornelia Polyak Loss of Kmt2c or Kmt2d drives brain metastasis via KDM6A-dependent upregulation of MMP3 Nat Cell Biol, (2024)

PubMed: 38926506

Applications

WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KMT2D/ALL1) Polyclonal Antibody, Unconjugated (orb184541) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.