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KLRK1 Antibody

SKU: orb1247953

Description

KLRK1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Lymph Node and Human Spleen lysates (calculated MW of 25.3kDa according to NP_031386.2). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1;104(9):2858-66. Epub 2004 Jul 6; PMID: 15238421). Recommended concentration: 0.1-0.3µg/mL. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Spleen, and Rat Thymus for this particular batch.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KVYSKEDQDLLK
TargetKLRK1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D

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KLRK1 Antibody

orb1247953 staining (0.3 ug/ml) of Human Spleen lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

KLRK1 Antibody (orb1247953)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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