KLRB1 Antibody / CD161

• Catalog Number: orb2635883
• Category: Antibodies
• Description: Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. T cells bearing natural killer receptors (NKRs) such as CD94 and CD161 are present in psoriasis. CD161 mediates NK cell activation and functions as an activating receptor. CD161 is a prototypic marker of NK cells, although it is also found on a subset of CD8+ T cells. The expression of NK receptors on CD8+ T cells can be considered a marker of cytotoxic effector T cells that are expanded in vivo after antigenic activation leading to extensive proliferation. The transcription, mRNA accumulation, and surface expression of CD161, a molecule involved in triggering cytotoxicity, is specifically upregulated by IL-12.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-225) of human KLRB1 protein was used as the immunogen for the CD161 antibody.
• UniProt ID: Q12918
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the CD161 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: KLRB1/8910
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Western blot testing of human spleen tissue lysate with CD161 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human prostate tissue with CD161 antibody (clone KLRB1/8910). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD161 antibody (clone KLRB1/8910) as confirmation of integrity and purity.