KLF8 antibody

• Catalog Number: orb18394
• Category: Antibodies
• Description: Goat polyclonal antibody to KLF8
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Porcine
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 39.3; 27.2
• Target: KLF8 / BKLF3
• Entrez: 11279
• Protein Sequence: VDMDKLINNLEVQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KLF8 antibody, anti BKLF3 antibody, anti Krup
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al. J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended for use at 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18394 staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18394 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.