KLF8 Antibody

SKU: orb1247954

Description

KLF8 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended concentration: 1-3µg/mL.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: VDMDKLINNLEVQ-C
• Target: KLF8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KLF8, BKLF3, Kruppel-like factor 8, RP13-1021K9.1, DKFZp686O08126, DXS741, MGC138314, ZNF741, zinc finger protein 741, basic kruppel-like factor 3

orb1247954 staining (2 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247954 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KLF8

UniProt

O95600

RefSeq (Protein):NP_001152768.1, NP_009181.2