KLF8 Antibody

• Catalog Number: orb1247954
• Category: Antibodies
• Description: KLF8 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: VDMDKLINNLEVQ-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KLF8
• UniProt ID: O95600
• NCBI: NP_009181.2, NP_001152768.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: KLF8, BKLF3, Kruppel-like factor 8, RP13-1021K9.1,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247954 staining (2 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247954 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.