KLF17 Antibody

• Catalog Number: orb2635183
• Category: Antibodies
• Description: Kruppel-like factors (KLFs) comprise a family of evolutionarily conserved zinc finger-containing transcription factors with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. Individual members of the Sp1-like/KLF family can function either as activators or repressors, depending on which promoter they bind and which co-regulators they interact with. KLF17 (Kruppel-like factor 17), whose alternative names include ZNF393 (zinc finger protein 393) or zfp393, is a 389 amino acid nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family. Expressed in testis and ovary, KLF17 may function as a germ cell-specific transcription factor involved in oocyte development and spermatid differentiation. Containing three C2H2-type zinc fingers which bind G/C-rich sites, KLF17 activates transcription from CACCC-box elements.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human KLF17protein was used as the immunogen for the KLF17 antibody.
• UniProt ID: Q5JT82
• Tested applications: FACS, IF, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Application notes: Optimal dilution of the KLF17 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-KLF17-1G2
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This KLF17 antibody is available for research use only.
• Note: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using KLF17 antibody (clone PCRP-KLF17-1G2). These results demonstrate the foremost specificity of the PCRP-KLF17-1G2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with KLF17 antibody (blue, clone PCRP-KLF17-1G2), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free KLF17 antibody (clone PCRP-KLF17-1G2) as confirmation of integrity and purity.