KLF10 Rabbit Polyclonal Antibody

SKU: orb1974706

Description

KLF10 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Epigenetics and Nuclear Signaling, Transcription Factors

Images & Validation

• Tested Applications: ICC
• Dilution Range: ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TIEG1 (401-480/480aa)
• Target: KLF10
• Molecular Weight: 53 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGR-alpha; EGRA; TIEG; TIEG1; EGR[a]; Egral; Gdnfif; TIEG-1; mGIF; KLF10_HUMAN; KLF10; Transforming growth factor-beta-inducible early growth response protein 1 (TGFB-inducible early growth response protein 1 | TIEG-1); KLF10_MOUSE; GDNF-inducible factor; Transcription factor GIF (mGIF); KLF10_RAT; Zinc finger transcription factor homolog CPG20; Cpg20;

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KLF10) polyclonal Antibody, Unconjugated (orb1974706) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

KLF10

UniProt

Q13118