Klenow Fragment

Catalog Number: orb94056

Catalog Number	orb94056
Category	Tools
Description	Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Clonality	Recombinant
Form/Appearance	liquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)
Concentration	5 units/µl
Purity	The enzyme is greater than 98% pure as indicated by SDS-
Application notes	Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.
Storage	store at -20 °C
Note	For research use only

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