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Catalog Number | orb94056 |
---|---|
Category | Tools |
Description | Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. |
Clonality | Recombinant |
Concentration | 5 units/µl |
Form/Appearance | liquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol) |
Purity | The enzyme is greater than 98% pure as indicated by SDS- |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends. |
Expiration Date | 12 months from date of receipt. |