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Klenow Fragment

Klenow Fragment

Catalog Number: orb94056

DispatchUsually dispatched within 5-10 working days
$ 330.00
Catalog Numberorb94056
CategoryTools
DescriptionKlenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
ClonalityRecombinant
Concentration5 units/µl
Form/Appearanceliquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)
PurityThe enzyme is greater than 98% pure as indicated by SDS-
Storagestore at -20 °C
NoteFor research use only
Application notesFill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.
Expiration Date12 months from date of receipt.