KL Antibody

• Catalog Number: orb1239544
• Category: Antibodies
• Description: KL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-KLOTHO antibody (orb1239544) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO. The immunogen is located within amino acids 410-460 of KLOTHO.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 116kDObserved: 116kD
• Target: KL
• UniProt ID: Q9UEF7
• NCBI: NP_004786
• Storage: KLOTHO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: KLOTHO Antibody: Klotho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: KLOTHO orb1239544 (2 µg/mL), KLOTHO, competitor antibody (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO orb1239544 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 72kD.

Western Blot Validation in Rat Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: KLOTHO orb1239544 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (orb1239544) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of KLOTHO in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with orb1239544 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (orb1239544) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.