KIF5A Antibody

SKU: orb1239543

Description

KIF5A Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5.The immunogen is located within amino acids 880 - 930 of KIF5.
• Target: KIF5A
• Molecular Weight: Predicted: 114 kDa Observed: 116 kDa
• Purification: KIF5 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: KIF5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

KIF5 Antibody: NKHC, MY050, SPG10, D12S1889, NKHC1, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, NKHC

Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 µg/mL.

Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 µg/mL.

Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 µg/mL.

Immunohistochemistry of KIF5 in mouse brain tissue with KIF5 antibody at 5 µg/ml.

Immunofluorescence of KIF5 in mouse brain tissue with KIF5 antibody at 20 µg/mL. Red: KIF5 Antibody (orb1239543) Blue: DAPI staining.

Quick Database Links

Gene Symbol

KIF5A

UniProt

Q12840

RefSeq:Q12840