Kidins220 Rabbit Polyclonal Antibody

SKU: orb100188

Description

Kidins220 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Eph Eeceptors, Kinases

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Kidins220 (651-750/1771aa)
• Target: KIDINS220
• Molecular Weight: 196 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Ankyrin repeat-rich membrane-spanning protein; arms; KDIS_HUMAN; Kidins220; Kinase D interacting substance of 220 kDa; Kinase D-interacting substrate of 220 kDa; rgd 619949.

Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Kidins220 antibody (orb100188), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA (green).

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Kidins220 Polyclonal Antibody, Unconjugated (orb100188) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

KIDINS220

UniProt

Q9ULH0