Ki67/MKI67 Antibody

• Catalog Number: orb180724
• Category: Antibodies
• Description: Ki67/MKI67 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunofluorescence, 5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 358694 MW
• UniProt ID: P46013
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Antigen KI-67;MKI67;
• Note: For research use only
• Application notes: WB: The detection limit for Ki67 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of Ki67 using anti-Ki67 antibody.Ki67 was detected in immunocytochemical section of HeLa cells.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

WB analysis of Ki67 using anti-Ki67 antibody.Lane 1:HELA Cell;2:MCF-7 Cell; 3:COLO320 Cell;4:HEPG2 Cell;5:SKOV Cell.

IF analysis of Ki67 using anti-Ki67 antibody.Ki67 was detected in paraffin-embedded section of human colon organoid tissue.

IHC analysis of Ki67 using anti-Ki67 antibody. Ki67 was detected in paraffin-embedded section of human tonsil tissues.