KI-67/MKI67 Antibody

• Catalog Number: orb1294315
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Ki-67
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200
• Conjugation: Unconjugated
• Target: KI-67 / MKI67
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Ki-67 antibody - at 1/500 dilution, Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA an hour before being incubated with orb1294315 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 with orb1294315 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 with orb1294315 at 1/200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294315 Anti-Ki-67 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.