Ki-67 Rabbit Polyclonal Antibody

• Catalog Number: orb500812
• Category: Antibodies
• Description: Ki-67 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Ki-67 (1231-1330/3256aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100, IF=1:300-800
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 358 kDa
• Target: MKI67
• UniProt ID: P46013
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Antigen identified by monoclonal antibody Ki 67; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb500812) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb500812) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.