KI-67 Antibody

• Catalog Number: orb1735092
• Category: Antibodies
• Description: KI-67 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide /Ki-67
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:50 – 1:100
• Conjugation: Unconjugated
• Target: KI-67
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1735092 overnight at 4°C. Lysates at 35 μg per lane. Anti Ki-67 antibody - at 1/500 dilution.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 antibody with orb1735092 at 1/100.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1735092 anti-Ki-67 antibody at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.