Keratin 36 Rabbit Polyclonal Antibody

SKU: orb184679

Description

Keratin 36 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Keratin 36 (301-400/467aa)
• Target: KRT36
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HA6; Hair keratin; Hair keratin, type I Ha6; HHA6; HKA6; K36; Keratin; Keratin hair acidic 6; Keratin type I cuticular Ha6; keratin, hair, acidic, 6; Keratin, hard, type I; Keratin, hard, type I, 6; keratin, type I cuticular Ha6; Keratin-36; Krt36; KRT36_HUMAN; KRTHA6; type I cuticular Ha6; type I Ha6.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Keratin 36) Polyclonal Antibody, Unconjugated (orb184679) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: HL-60 Cell (Human) Lysate at 30 ug, Primary: Anti-Keratin (orb184679) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

KRT36

UniProt

O76013