Keratin 13 Antibody

• Catalog Number: orb1246634
• Category: Antibodies
• Description: Keratin 13 Antibody
• Target: KRT13
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TSNASGRRTSDVRRP
• UniProt ID: P13646
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 55kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KRT13, keratin 13, CK13, K13, MGC161462, MGC3781,
• Research Area: Cell Biology
• Note: For research use only
• NCBI: NP_705694.2

orb1246634 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246634 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.