KDM6B Antibody

• Catalog Number: orb1239526
• Category: Antibodies
• Description: KDM6B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-JMJD3 antibody (orb1239526) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 180Observed: 180 kD
• Target: KDM6B
• UniProt ID: O15054
• NCBI: EAW90126
• Storage: JMJD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-spe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD3 orb1239526 (1 µg/mL), JMJD3 orb1260760, (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.25 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in K562 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.

Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.

Western Blot Validation in HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL, Lane 2: 0.5 µg/mL Lane 3: 0.25 µg/mL with blocking peptide.

Immunocytochemistry Validation of JMJD3 in K562 Cells. Immunocytochemical analysis of K562 cells using anti-JMJD3 antibody (orb1239526) at 2.5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with orb1239526 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).