KDM1/LSD1 Rabbit Polyclonal Antibody

• Catalog Number: orb157742
• Category: Antibodies
• Description: KDM1/LSD1 Rabbit Polyclonal Antibody
• Target: KDM1A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human KDM1 (301-400/852aa)
• UniProt ID: O60341
• MW: 94 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Amine oxidase(flavin containing) domain 2; Amine o
• Research Area: Cell Biology
• Note: For research use only

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KDM1/LSD1) polyclonal Antibody, Unconjugated (orb157742) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Testis (Rat) Lysate at 40 ug, Lane 5: Hela (Human) Cell Lysate at 30 ug, Lane 6: 293T (Human) Cell Lysate at 30 ug, Lane 7: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-KDM1/LSD1 (orb157742) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Sample: placenta (Mouse) Lysate at 40 ug, Primary: Anti-KDM1 (orb157742) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 94 kD, Observed band size: 94 kD.