KCNE1L Rabbit Polyclonal Antibody

SKU: orb1140

Description

KCNE1L Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/test
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KCNE1L (11-110/142aa)
• Target: KCNE5
• Molecular Weight: 15 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AMME syndrome candidate gene 2 protein; AMMECR2 protein; Cardiac voltage gated potassium channel accessory subunit 5; KCNE1 like; KCNE5; Mink; MinK like protein; Potassium voltage gated channel subfamily E member 1 like protein; Potassium voltage gated channel, Isk related family, member 1 like; Voltage gated potassium channel accessory subunit 5; KCE1L_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-KCNE1L antibody (orb1140), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

KCNE5

UniProt

Q9UJ90