KCC2 Rabbit Polyclonal Antibody

SKU: orb184496

Description

KCC2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KCC2 (851-950/1139aa)
• Target: SLC12A5
• Molecular Weight: 123 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DEE34; EIEE34; EIG14; KCC2; hKCC2; mKIAA1176; S12A5_HUMAN; SLC12A5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2 (hKCC2); Neuronal K-Cl cotransporter; KIAA1176; S12A5_MOUSE; K-Cl cotransporter 2 (mKCC2); S12A5_RAT; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2 (rKCC2); solute carrier family 12 member 5; solute carrier family 12 (potassium/chloride transporter), member 5; K-Cl cotransporter 2

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-KCC2 Polyclonal Antibody, Unconjugated (orb184496) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC12A5

UniProt

Q9H2X9