KAT3A / CBP Rabbit Polyclonal Antibody

• Catalog Number: orb10257
• Category: Antibodies
• Description: KAT3A / CBP Rabbit Polyclonal Antibody
• Target: CREBBP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CBP (1-200/2442aa)
• UniProt ID: Q92793
• RRID: AB_10746265
• MW: 265 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CBP; CBP_HUMAN; CREB binding protein; CREB-binding
• Research Area: Bromodomains (BRDs), Cell Biology, Coactivators an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-CBP antibody (orb10257), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CBP) Polyclonal Antibody, Unconjugated (orb10257) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.