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KANK1 Mouse Monoclonal Antibody
Catalog Number: orb783357
| Catalog Number | orb783357 |
|---|---|
| Category | Antibodies |
| Description | KANK1 Mouse Monoclonal Antibody |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | Y6S1 |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG1,k |
| Immunogen | Recombinant human KANK1. |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:25, IHC-F=1:25, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 65 kDa |
| Target | KANK1 |
| UniProt ID | Q14678 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | ANKRD 15; Ankyrin repeat domain 15; Ankyrin repeat Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Human heart tissue sections), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KANK1) Monoclonal Antibody, Unconjugated (orb783357) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Sample: Lane 1: Jurkat cell lysates, Lane 2: K562 cell lysates, Primary: Anti-KANK1 (orb783357) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 150 kD.
- Item 1 of 2
KANK1 monoclonal antibody [orb1677052]
IHC, WB
Human
Mouse
Monoclonal
Unconjugated
200 μl, 100 μl, 50 μl
