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Catalog Number | orb1939093 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Mouse |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | O15054 |
MW | 176632 Da |
Tested applications | IF, IHC-P, WB |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB10077 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Lysine-specific demethylase 6B, 11411-, JmjC domai Read more... |
Note | For research use only |
NCBI | NP_001073893.1 |
Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-JMJD3 (N-term) Pab in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.