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Catalog Number | orb1527741 |
---|---|
Category | Antibodies |
Description | JARID1B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | between 1494 and 1544 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | JARID1B |
UniProt ID | Q9UGL1 |
NCBI | NP_006609.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | PLU-1; retinoblastoma-binding protein 2 homolog 1; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody orb1527741 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody orb1527741.
Detection of human JARID1B by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody (orb1527741) used at 1: 500 (2µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
FC, ICC, IF, IHC, WB | |
Bovine, Canine, Equine, Hamster, Rabbit | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P | |
Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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