Jak3 Rabbit Polyclonal Antibody

• Catalog Number: orb6252
• Category: Antibodies
• Description: Jak3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Jak3 (601-700/1124aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 125 kDa
• Target: JAK3
• UniProt ID: P52333
• RRID: AB_10941864
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: JAK 3; JAK L; JAK3 HUMAN; JAKL; Janus kinase 3(a p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-Jak3 antibody (orb6252), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-Jak3 antibody (orb6252), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 0.2 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Rat Lymph node tissue lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human Hela cell lysates, Lane 5: Human Molt-4 cell lysates, Primary: Anti-Jak3 (orb6252) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kDa, Observed band size: 115 kDa.

Sample: SP2/0 Cell (Mouse) Lysate at 40 ug, Jurkat Cell (Human) Lysate at 40 ug, K562 Cell (Human) Lysate at 40 ug, Primary: Anti-Jak3 (orb6252) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Jak3 Polyclonal Antibody, Unconjugated (orb6252) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.