JAK2 antibody

• Catalog Number: orb10948
• Category: Antibodies
• Description: JAK2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human JAK2 (601-700/1132 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: JAK2
• UniProt ID: O60674
• RRID: AB_10746397
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Tyrosine protein kinase JAK2; JAK 2; JAK-2; JAK2_H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Truong, Anh Duc et al. Chicken IL-26 regulates immune responses through the JAK/STAT and NF-κB signaling pathways Dev Comp Immunol, 73, (2017)

PubMed: 28259699

Flow cytometry of mouse splenocytes(blue).Primary Antibody Dilution:1μl in 100 μL1X PBS containing 0.5% BSA(green).

Flow cytometry of HL-60.Primary Antibody (green line): Rabbit Anti-phospho-JAK2 (Tyr221) antibody; Dilution:1μg /10^6 cells.

IHC-P analysis of Rat liver using JAK2 Polyclonal Antibody at 1:500 dilution.

IHC-P analysis of mouse lung using JAK2 Polyclonal Antibody at 1:200 dilution.

Western blot:Lane 1:K562 (Human) Cell;Lane 2:BV-2 (Mouse) Cell;Primary:Anti-JAK2 at 1:1000 dilution.