ITM2B antibody

• Catalog Number: orb20142
• Category: Antibodies
• Description: Goat polyclonal antibody to ITM2B
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 30.3
• Target: ITM2B
• Entrez: 9445
• Protein Sequence: HDKETYKLQRRETIK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ITM2B antibody, anti integral membrane protei
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 33kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20142 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of HepG2 cell lysate using ITM2B antibody

Immunohistochemical staining of Human Small Intestine using ITM2B antibody

Immunofluorescence analysis of A431 cells using ITM2B antibody

Immunofluorescence analysis of HeLa cells using ITM2B antibody

Flow cytometric analysis of HeLa cells using ITM2B antibody