ITM2A Rabbit Polyclonal Antibody

• Catalog Number: orb1572
• Category: Antibodies
• Description: ITM2A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ITM2A (21-120/263aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: ITM2A
• UniProt ID: O43736
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BRICD2A; BRICHOS domain containing 2A; E25 protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): 293T Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ITM2A/AF488 Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ITM2A (orb1572) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 35 kD.

Sample: K562 Cell (Human) Lysate at 40 ug, Primary: Anti-ITM2A (orb1572) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.