ITGAL Antibody

• Catalog Number: orb1671720
• Category: Antibodies
• Description: ITGAL Antibody
• Clonality: Recombinant
• Clone Number: MHM24
• Tested applications: FA, FC
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: ITGAL
• UniProt ID: P20701
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Integrin alpha-L, CD11 antigen-like family member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Jurkat cells using anti-CD11a (HM24). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells stained with the chimeric mouse IgG version of MHM24 (1) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom. 1- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-CD11a antibody MHM24. Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (.1; isotype control - black line) or the mouse IgG1 version of MHM24 (orb1671720 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.