ISX/Intestine specific homeobox Rabbit Polyclonal Antibody

• Catalog Number: orb184451
• Category: Antibodies
• Description: ISX/Intestine specific homeobox Rabbit Polyclonal Antibody
• Target: ISX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox (81-180/245aa)
• UniProt ID: Q2M1V0
• MW: 27 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DKFZp781N2395; Intestine-specific homeobox; Isx; I
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (orb184451) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Lovo (Human) Cell Lysate at 30 ug, Lane 2: SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-ISX/Intestine specific homeobox (orb184451) at 1:500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: small intestine (Mouse) Lysate at 40 ug, large intestine (Mouse) Lysate at 40 ug, Primary: Anti-ISX/Intestine specific homeobox (orb184451) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.