Isotyping Kit

• Catalog Number: orb342280
• Category: Assays and Kits
• Description: Isotyping Kit
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 Kit
• Purity: Biorbyt' IsoMax Rapid Mouse Monoclonal Isotyping Kit is a rapid (5 minute) lateral flow assay with sensitivity equal to ELISA for monoclonal antibody class and subclass determination. The assay is designed to test antibody present in tissue culture supernatant or mouse ascites fluid. This kit contains 5 packs of 2 cassettes each sufficient for testing up to 5 samples. Please read the entire product insert prior to use.
• Tested applications: ELISA
• Application notes: Each kit contains 5 packs of 2 cassettes each: one cassette for detecting IgG1, IgG2a and IgG2b; the other cassette for detecting IgG3, IgA and IgM. When a properly diluted sample containing a specific isotype antibody is added to the sample well, a specific class and subclass soluble complex is formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and anti-class-specific antibody impregnated membrane. A control line will appear on the membrane in the region marked ''C'' on the cassette indicating a successful run (positive internal control). Typically when antibodies are applied at a concentration of 10 ng/mL the best results are obtained by reading the cassette 5 to 10 minutes after application. Results should NOT be read after 10 minutes.
• Storage: See kit insert for complete instructions.
• Alternative names: Isotyping kit, mouse subclass determination, IgG i
• Note: For research use only

IsoMax™ Rapid Mouse Monoclonal Isotyping Kit results showing that for ascites fluid the darker red line indicates the class or subclass of antibody present. Often an additional weaker red line may appear indicating the presence of host (endogenous) serum immunoglobulins present in the ascites.

Biorbyt's IsoMax™ Isotyping Kit compares to an ELISA-format isotyping assay. Results demonstrate outstanding correlation between these two assay formats. The ELISA required controls, an ELISA plate and substrate and took 8 hours of hands-on time to coat the plates, block and subsequently perform the assay using 12 different antibodies. The IsoMax™ Isotyping Kit pipetting time was 10 minutes; all 20 assays were run simultaneously. Each cassette was read at 5 minutes. The total hands-on time for the IsoMax™ Kit, including scoring of the results, was under twenty minutes.