Islet-2 rabbit pAb Antibody

SKU: orb765526

Description

Rabbit polyclonal antibody to Islet-2

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ISL2. AA range:161-210
• Molecular Weight: 40
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-ISL2 antibody, anti-Insulin gene enhancer protein ISL-2 antibody, anti-Islet-2 antibody

Western Blot analysis of various cells using Islet-2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of lysates from 293 cells, using ISL2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).