ISG15 Antibody

• Catalog Number: orb371684
• Category: Antibodies
• Description: ISG15 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ISG15 recombinant protein (Position: G2-G157). Human ISG15 shares 65.8% amino acid (aa) sequence identity with mouse ISG15.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 17888 MW
• UniProt ID: P05161
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ubiquitin-like protein ISG15;Interferon-induced 15
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ISG15 using anti-ISG15 antibody.Lane 1:22RV1 cell; 2:HELA cell.

IHC analysis of ISG15 using anti-ISG15 antibody.ISG15 was detected in a paraffin-embedded section of human pancreatic cancer tissue.