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IRS2 Antibody

Catalog Number: orb1262941

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1262941
CategoryAntibodies
DescriptionIRS2 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-P, WB
ReactivityHuman
IsotypeRabbit Ig
ImmunogenThis IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MW137 kDa
TargetIRS2
UniProt IDQ9Y4H2
NCBIQ9Y4H2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied in PBS with 0.09% (W/V) sodium azide.
Alternative namesInsulin receptor substrate 2, IRS-2, IRS2
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NoteFor research use only
Application notesFor WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
Expiration Date12 months from date of receipt.
IRS2 Antibody

Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

IRS2 Antibody

Fluorescent image of Hela cell stained with IRS2 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).IRS2 immunoreactivity is localized to Cytoplasm significantly.

IRS2 Antibody

Western blot analysis of anti-IRS2 Antibody in 293 cell line lysates (35 ug/lane)

IRS2 Antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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    FC,  IF,  IHC-Fr,  IHC-P

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    50 μl, 100 μl, 200 μl
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    FC,  IF,  IHC-Fr,  IHC-P

    Mouse, Rat

    Human, Mouse, Rat

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    Unconjugated

    100 μl, 50 μl, 200 μl