You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1262941 |
---|---|
Category | Antibodies |
Description | IRS2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 137 kDa |
Target | IRS2 |
UniProt ID | Q9Y4H2 |
NCBI | Q9Y4H2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Insulin receptor substrate 2, IRS-2, IRS2 Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of Hela cell stained with IRS2 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).IRS2 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-IRS2 Antibody in 293 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
FC, ICC, IF, IHC-Fr, IHC-P | |
Equine, Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |