IRS1 Antibody

• Catalog Number: orb215977
• Category: Antibodies
• Description: IRS1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 131591 MW
• UniProt ID: P35568
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Insulin receptor substrate 1;IRS-1;IRS1;
• Note: For research use only
• Application notes: WB: The detection limit for IRS1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Intestine Tissue using Anti-IRS1 antibody.

IHC(P) analysis of Rat Intestine Tissue using Anti-IRS1 antibody.

IHC(P) analysis of Rat Skeletal Muscle Tissue using Anti-IRS1 antibody.

IHC(P) analysis of Human Intestinal Cancer Tissue using Anti-IRS1 antibody.

IHC(P) analysis of Human Lung Cancer Tissue using Anti-IRS1 antibody.

WB analysis of Recombinant Human IRS1 Protein 0.5ng using Anti-IRS1 antibody.

WB analysis using Anti-IRS1 antibody.Lane 1: A549 Cell;2:MM453 Cell;3: JURKAT Cell.