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Catalog Number | orb184392 |
---|---|
Category | Antibodies |
Description | IRS1 (phospho-Ser616) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser616 (PM(p-S)PG) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 131 kDa |
Target | phospho-IRS1 (Ser616) |
UniProt ID | P35568 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | IRS1 (phospho S616); p-IRS1 (phospho S616); IRS1_H Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of HepG2.Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser616) antibody; Dilution:1μg /10^6 cells.
ICC analysis of A549 cell using phospho-IRS1 (Ser616) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of rat uterus using phospho-IRS1 Ser616 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of Mouse brain using phospho-IRS1 Ser616 Polyclonal Antibody at 1:400 dilution.
Western blot:Lane 1:Mouse Cerebrum;Lane 2:Mouse Cerebellum;Primary:Anti-phospho-IRS1 (Ser616) at 1:1000 dilution.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
FC, ICC, IF | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |
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