IRGM Antibody

SKU: orb1239615

Description

IRGM Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM.The immunogen is located within amino acids 30 - 80 of IRGM.
• Target: IRGM
• Molecular Weight: Predicted: 20 kDa Observed: 20 kDa
• Purification: IRGM Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IRGM Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

IRGM Antibody: IFI1, IRGM1, LRG47, LRG-47, IFI1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1

Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 µg/ml.

Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 µg/mL. Green: IRGM Antibody (orb1239615) Blue: DAPI staining.

Quick Database Links

Gene Symbol

IRGM

UniProt

A1A4Y4

RefSeq:AAI28169