IRGC Rabbit Polyclonal Antibody

• Catalog Number: orb184388
• Category: Antibodies
• Description: IRGC Rabbit Polyclonal Antibody
• Target: IRGC
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human IRGC (301-400/463aa)
• UniProt ID: Q6NXR0
• MW: 50 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: F630044M05Rik; Gm1102; Gm474; IIGP5; IIGP5_HUMAN;
• Note: For research use only

Sample: A549 Cell (Human) Lysate at 30 ug, Primary: Anti-IRGC (orb184388) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Tissue/Cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IRGC Polyclonal Antibody, Unconjugated (orb184388) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.