IRAK2 Antibody

• Catalog Number: orb1238892
• Category: Antibodies
• Description: IRAK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.The immunogen is located within amino acids 530 - 580 of IRAK-2.
• Concentration: 1 mg/ml
• Dilution range: IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: IRAK2
• UniProt ID: O43187
• NCBI: AF026273
• Storage: IRAK-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IRAK-2 Antibody: IRAK-2, Interleukin-1 receptor-as
• Note: For research use only
• Application notes: IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 µg/mL.

Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 µg/mL.

Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 µg/mL.

Immunofluorescence of IRAK-M in human spleen tissue with IRAK M antibody at 20 µg/mL. Green: IRAK-2 Antibody (orb1238892) Blue: DAPI staining.