IRAK3 Antibody

SKU: orb1239522

Description

IRAK3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M.The immunogen is located within the last 50 amino acids of IRAK-M.
• Target: IRAK3
• Molecular Weight: Predicted: 66 kDa Observed: 70 kDa
• Purification: IRAK-M Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAK-3

Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 µg/mL.

Immunohistochemistry of IRAK-M in human spleen tissue with IRAK-M antibody at 5 µg/mL.

Immunofluorescence of IRAK-M in Rat Liver tissue with IRAK-M antibody at 10 µg/mL.

Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/mL.

Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 µg/mL. Red: IRAK-M Antibody (orb1239522) Blue: DAPI staining.

Quick Database Links

Gene Symbol

IRAK3

UniProt

Q9Y616

RefSeq (Protein):NP_009130