IQGAP1 Rabbit Polyclonal Antibody

• Catalog Number: orb10931
• Category: Antibodies
• Description: IQGAP1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IQGAP1 (1451-1550/1657aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 189 kDa
• Target: IQGAP1
• UniProt ID: P46940
• RRID: AB_10751109
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IQ motif containing GTPase activating protein 1; K
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-IQGAP1 antibody (orb10931), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.