Interleukin-10 IL10 Antibody

• Catalog Number: orb76364
• Category: Antibodies
• Description: Interleukin-10 IL10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat IL-10 recombinant protein (Position: S19-N178).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat ELISA , 0.1-0.5μg/ml, Rat, -Western blot, 0.1-0.5μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 20426 MW
• UniProt ID: P29456
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-10;IL-10;Cytokine synthesis inhibitory
• Note: For research use only
• Application notes: By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

References

Jin, Jin et al. Corrigendum: Lipoxin A4 Inhibits NLRP3 Inflammasome Activation in Rats With Non-compressive Disc Herniation Through the JNK1/Beclin-1/PI3KC3 Pathway Front Neurosci, 14, 608184 (2020)

PubMed: 33312119

Western blot analysis of Lane 1: Recombinant Rat IL10 Protein 10ng, Lane 2: Recombinant Rat IL10 Protein 5ng, Lane 3: Recombinant Rat IL10 Protein 2.5ng using IL10 antibody