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Catalog Number | orb1262373 |
---|---|
Category | Antibodies |
Description | IL10 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Monkey |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 21 kDa |
Target | IL10 |
UniProt ID | P22301 |
NCBI | P22301 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Interleukin-10, IL-10, Cytokine synthesis inhibito Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of IL10 Antibody in MDA-MB435 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.