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Catalog Number | orb1125478 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Interleukin-10 (IL10) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Recombinant (Human) full length protein produced in E. coli |
Dilution range | ELISA: 1/400 - 1/1200 (as detection antibody, using a monoclonal antibody for capture). IHC-Fr: 1/400. IHC-P: 1/400. IP: 1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background. Neut: 1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. RIA: 1/4000. WB: 1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Buffer/Preservatives | Preservative: 0.01% Sodium Azide Constituents: Whole serum |
Note | For research use only |
Application notes | ELISA: 1/400 - 1/1200 (as detection antibody, using a monoclonal antibody for capture). IHC-Fr: 1/400. IHC-P: 1/400. IP: 1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background. Neut: 1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. RIA: 1/4000. WB: 1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule |
Expiration Date | 12 months from date of receipt. |
IHC-P | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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