Interferon alpha 2b Rabbit Polyclonal Antibody

• Catalog Number: orb13483
• Category: Antibodies
• Description: Interferon alpha 2b Rabbit Polyclonal Antibody
• Target: IFNA2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human IFN alpha 2b
• UniProt ID: P01563
• RRID: AB_10750818
• MW: 19 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interferon alpha-2b; alpha 2a interferon; alpha-2a
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Interferon alpha 2b) Polyclonal Antibody, Unconjugated (orb13483) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human Interferon alpha 2 protein, N-His, Primary: Anti-Interferon alpha 2b (orb13483) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19 kDa, Observed band size: 16 kDa.

Tissue/Cell: mouse liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFN-Alpha 2b/Interferon alpha 2/INFA2 Polyclonal Antibody, Unconjugated (orb13483) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.